KEMENTERIAN PENDIDIKAN TINGGI
MINISTRY OF HIGHER EDUCATION
MALAYSIA
PRGS FASA 1 TAHUN 2017
PRGS/1/2017/STG05/UKM/03/1
Bedside Rapid Point-of-care Biosensor Kit For Sepsis And Bacterial Infection Detection
Research Domain: Pure and Applied Science
Sub Domain: Biotechnology
PROJECT LEADER
Assoc. Prof. Dr. Tan Toh Leong
Associate Professor
Department of Emergency Medicine
Hospital Chancellor Tuanku Mukhriz
Universiti Kebangsaan Malaysia
sebastianttl@yahoo.co.uk
RESEARCH MEMBERS
NO NAME INSTITUTION FACULTY/SCHOOL/ CENTRE/UNIT
1 Prof. Dr. Lee Yook Heng UKM Faculty of Science and Technology
2 Assoc. Prof. Dr. Gan Kok Beng UKM Faculty of Engineering & Built Environment
3 Assoc. Prof. Dr. Nurul Izzaty Hassan UKM Faculty of Science and Technology
4 Assoc. Prof. Dr. Neoh Hui-min UKM UKM Medical Molecular Biology Institute
RESEARCH DURATION
2 years (15 August 2017 - 14 August 2019)
RESEARCH ABSTRACT
Bacterial sepsis is one of the leading causes of death in Malaysia, with a prevalence of 35% in Universiti Kebangsaan Malaysia Medical Center. Currently, bacterial sepsis is detected via bacterial cultures or the ELISA method. However, these methods require more than 24 hours to produce results. The secretory group IIA Phospholipase A2 (sPLA2- IIA) was found to have high sensitivity and specificity for bacterial infection diagnosis (~90%). This biomarker was successfully determined by our newly-developed electrochemical biosensor (based on first-of-its-kind enzymatic cascading reactions of three enzymes immobilised on acrylic-microspheres coated onto a screen-printed electrode). We had made this point-of-care testing (POCT) biosensor more user-friendly and small size. The POCT to achieve clinical diagnosis for on-site bacterial infection diagnosis in 15 minute. It enables risk stratification in early phase of bacterial sepsis to improve patient outcome.
RESEARCH OBJECTIVES
  1. To miniaturize a user-friendly alpha prototype device consisting of integrated electrode and portable handheld electronic reader for rapid detection of bacterial sepsis.
  2. To test the performance of the newly-fabricated alpha prototype
RESEARCH OUTPUT
  1. Intellectual Property (IP) :
    Proposed Achieve
    1 2

    • Patent (UKM.IKB.800-4/1/2701)
    • Trademark (UKM.IKB.800-4/1/2702)
  2. Product/Prototype: SepsiDotTM
    • 3-in-1 tri-enzymatic sceen-printed eletrode : Horseradish peroxidase, HRP, choline oxidase, ChOx and choline kinase, ChKinase.
    • Miniature portable potentiostat reader./li>
  3. Publication : Article in Indexed Journals
    • 3-in-1 tri-enzymatic sceen-printed eletrode : Horseradish peroxidase, HRP, choline oxidase, ChOx and choline kinase, ChKinase.
    • Miniature portable potentiostat reader./li>

    Conference Proceedings
    • PERSIDANGAN DAN EKSPO CIPTAAN INSTITUSI PENGAJIAN TINGGI ANTARABANGSA (PECIPTA) (2019)
AWARD
  • PERSIDANGAN DAN EKSPO CIPTAAN INSTITUSI PENGAJIAN TINGGI ANTARABANGSA (PECIPTA) 2019) – Silver Medal
RESEARCH IMPACT
  1. Development: a 10-minute rapid diagnostic kit to detect sepsis and bacterial infection.
  2. Novelty: one of its kind in the market, current options take more than a day for diagnosis.
  3. Portable, affordable and easy to use in any environment, including disaster and war zones.
APPENDIX
VIDEO
 
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